Generation of iPSC line from MYH7 R403L mutation carrier with severe hypertrophic cardiomyopathy and isogenic CRISPR/Cas9 corrected control

MYH7 is a major gene responsible for hypertrophic cardiomyopathy (HCM). From patient's skin fibroblasts, we derived an iPSC line (CDGEN1.16) harboring the heterozygous R403L mutation, hot-spot codon in HCM. We subsequently corrected mutated using CRISPR/Cas9 editing and obtained isogenic control (CDGEN1.16.40.5) preserving genomic background of patient. Both lines were pluripotent could be efficiently committed to beating cardiomyocytes (CM) suitable subsequent cell or pseudo-tissue study HCM pathology.